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The alarming picture of the post-Napoleonic period could not leave the young Roman priest, Gaspar Del Bufalo (1786-1837), indifferent. In his contemplation of God’s love for humanity expressed in Jesus’ bloody sacrifice, Gaspar understood the priceless value of each human person redeemed by His Precious Blood and sees in the power of this Blood the only anchor of salvation from many evils. Finding himself a prisoner in Bologna, he plans two Institutes to this end, one a men’s institute and the other a women’s. Both were to contribute to the reform of society, the first through popular missions and the second through effective Christian and devotional education, working with all their might that this blood not be shed in vain but that each person take advantage of it for his or her salvation.
The women's Institute begins in Acuto (FR) in the diocese of Anagni, through the work of Maria De Mattias who opened a school for the girls of the people. Later she gathers young people and married women, gives retreats, animates liturgies, promotes women who will be able to reform their families, the first cell of society. In a short time, other companions join her, and open schools in the small towns of the Pontifical State and the Kingdom of Naples. She wrote, “The purpose of this Institute is the same as that of the Blood of Christ.”
His holiness Pius IX grants the “decree of praise” after having learned of the Institute from the testimonial letters of the Bishops in whose dioceses the Adorers zealously dedicated themselves to the Christian and civic education of the poor girls.
The Sisters of Perpetual Adoration of the Precious Blood, founded in Steinerberg (Switzerland) and transferred to Gurtweil (Germany), join the Institute of the Adorers of Acuto, adopting its Rule and habit.
Maria De Mattias dies in Rome of tuberculosis at the age of 61. At that time, there were more than 200 Sisters in the Institute. Sixty-four schools had been opened, one of which was in London (1863 -1879).

A special General Chapter was held for the revision of the Constitution required by Vatican Council II. This chapter is prepared by an international commission, which involved all the Sisters of the Congregation. The principles emphasized by the Council in religious life were applied. “Return to our sources” was facilitated by the coinciding of the centennial of the death of the Foundress and discovery of many documents from the beginnings of the Institute in the Anagni diocesan archives.
The title of the Institute is changed from Sisters of the Most Precious Blood (Ad.PP.S.) to Sisters Adorers of the Blood of Christ (ASC).

150th anniversary of foundation.
This celebration, preceded by three years of preparation with in-depth study of the themes of adoration-mission, contributes to the development of the charism of the Congregation and the spirituality of the Precious Blood.
Definitive approval of the Constitution by the Congregation for Religious, after a twelve-year experimentation and an updated revision with the involvement of all the members of the Congregation.
Five Adorers of the Blood of Christ from the USA, missionaries in Liberia (Africa), are killed during the civil war. The Pope called them “Martyrs of charity.”
The three American provinces joined in a single United States Region. The headquarters of the US ASC administration is in St. Louis-Missouri.
The four Italian provinces also made the same choice by merging into a single Italian Region. The seat of the regional administration is the house of S. Giovanni in Laterano, in Rome.